nsrR [2017-8-11 16:0:3]

nitric oxide-responsive regulator

locus: BSU09380

pI: 7.75

mw: 16.47 kDa

protein length: 146 aa

gene length: 438 bp

function: repression of ResD-ResE-dependent genes in the absence of nitric oxide (NO)

product: transcriptional repressor

essential: no

synonyms: yhdE

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 1,013,411 → 1,013,851

The protein

[SW|Cofactors]

• [4Fe-4S] cluster, required for DNA-binding [Pubmed|21091510]

Effectors of protein activity

• nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets [Pubmed|19006327]
• [4Fe-4S]-NsrR binds around the -35 element of the ''[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]'' promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO [Pubmed|21091510]

Expression and Regulation

Operons

• genes: [gene|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]
  description: [Pubmed|10322020]

Biological materials

Mutant

• MGNA-A692 (yhdE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/692 NBRP B. subtilis, Japan]

References

Reviews

• 23046954,16261196,20167493

The [SW|NsrR regulon]

• 22287527

Other original publications

• 16885456,24214949,17293416,21091510,19006327,18989365,28439033